Journal: OncoTargets and therapy

Article Title: Roles Of EAAT1, DHFR, And Fetuin-A In The Pathogenesis, Progression And Prognosis Of Chondrosarcoma

doi: 10.2147/OTT.S222426

Figure Lengend Snippet: EAAT1, DHFR, and fetuin-A expression in chondrosarcoma (CS) and chondroma tissues. Representative patterns of EAAT1 expression in CS (positive [ A ] and negative [ B ]) and chondroma (positive [ G ] and negative [ H ]), DHFR expression in CS (positive [ C ] and negative [ D ]) and chondroma (positive [ I ] and negative [ J ]), and fetuin-A expression in CS (positive [ E ] and negative [ F ]) and chondroma (positive [ K ] and negative [ L ]) are shown (400×). Representative patterns of different staining intensities are shown: intensity 0 ( M ), intensity 1 ( N ), intensity 2 ( O ), intensity ( P ).

Article Snippet: Following a 20-min sitting in sodium citrate buffer (10 mM sodium citrate, 0.05% Tween 20, pH 6.0) at room temperature for 20 mins, the sections were incubated with rabbit anti-human EAAT1 [1:50], DHFR [1:50], or fetuin-A [1:50] antibodies (Boster Biological Technology Co., Ltd., California, USA) for 2 hrs and then rinsed with 1× phosphate buffered saline three times for 5 mins each.

Techniques: Expressing, Staining

Journal: OncoTargets and therapy

Article Title: Roles Of EAAT1, DHFR, And Fetuin-A In The Pathogenesis, Progression And Prognosis Of Chondrosarcoma

doi: 10.2147/OTT.S222426

Figure Lengend Snippet: EAAT1, DHFR, And Fetuin-A Expression In CS And Chondroma

Article Snippet: Following a 20-min sitting in sodium citrate buffer (10 mM sodium citrate, 0.05% Tween 20, pH 6.0) at room temperature for 20 mins, the sections were incubated with rabbit anti-human EAAT1 [1:50], DHFR [1:50], or fetuin-A [1:50] antibodies (Boster Biological Technology Co., Ltd., California, USA) for 2 hrs and then rinsed with 1× phosphate buffered saline three times for 5 mins each.

Techniques: Expressing

Journal: OncoTargets and therapy

Article Title: Roles Of EAAT1, DHFR, And Fetuin-A In The Pathogenesis, Progression And Prognosis Of Chondrosarcoma

doi: 10.2147/OTT.S222426

Figure Lengend Snippet: Association Of EAAT1, DHFR, And Fetuin-A Expression With Clinicopathological Characteristics Of CS

Article Snippet: Following a 20-min sitting in sodium citrate buffer (10 mM sodium citrate, 0.05% Tween 20, pH 6.0) at room temperature for 20 mins, the sections were incubated with rabbit anti-human EAAT1 [1:50], DHFR [1:50], or fetuin-A [1:50] antibodies (Boster Biological Technology Co., Ltd., California, USA) for 2 hrs and then rinsed with 1× phosphate buffered saline three times for 5 mins each.

Techniques: Expressing

Journal: OncoTargets and therapy

Article Title: Roles Of EAAT1, DHFR, And Fetuin-A In The Pathogenesis, Progression And Prognosis Of Chondrosarcoma

doi: 10.2147/OTT.S222426

Figure Lengend Snippet: Associations of EAAT1, DHFR, and fetuin-A expression with survival, represented by Kaplan–Meier curves, in patients with CS. ( A ) EAAT1 expression (mean survival, positive 30.48 vs. negative 41.64 months; p = 0.017). ( B ) DHFR expression (mean survival, positive 29.77 vs. negative 40.56 months; p = 0.015). ( C ) Fetuin-A expression (mean survival, positive 31.07 vs. negative 40.66 months; p = 0.022).

Article Snippet: Following a 20-min sitting in sodium citrate buffer (10 mM sodium citrate, 0.05% Tween 20, pH 6.0) at room temperature for 20 mins, the sections were incubated with rabbit anti-human EAAT1 [1:50], DHFR [1:50], or fetuin-A [1:50] antibodies (Boster Biological Technology Co., Ltd., California, USA) for 2 hrs and then rinsed with 1× phosphate buffered saline three times for 5 mins each.

Techniques: Expressing

Journal: OncoTargets and therapy

Article Title: Roles Of EAAT1, DHFR, And Fetuin-A In The Pathogenesis, Progression And Prognosis Of Chondrosarcoma

doi: 10.2147/OTT.S222426

Figure Lengend Snippet: Relationship Of Clinicopathological Characteristics With Survival In Patients With CS

Article Snippet: Following a 20-min sitting in sodium citrate buffer (10 mM sodium citrate, 0.05% Tween 20, pH 6.0) at room temperature for 20 mins, the sections were incubated with rabbit anti-human EAAT1 [1:50], DHFR [1:50], or fetuin-A [1:50] antibodies (Boster Biological Technology Co., Ltd., California, USA) for 2 hrs and then rinsed with 1× phosphate buffered saline three times for 5 mins each.

Techniques:

Journal: OncoTargets and therapy

Article Title: Roles Of EAAT1, DHFR, And Fetuin-A In The Pathogenesis, Progression And Prognosis Of Chondrosarcoma

doi: 10.2147/OTT.S222426

Figure Lengend Snippet: Multivariate Cox Regression Analysis Of Clinicopathologic Characteristics With Overall Survival In Patients With CS

Article Snippet: Following a 20-min sitting in sodium citrate buffer (10 mM sodium citrate, 0.05% Tween 20, pH 6.0) at room temperature for 20 mins, the sections were incubated with rabbit anti-human EAAT1 [1:50], DHFR [1:50], or fetuin-A [1:50] antibodies (Boster Biological Technology Co., Ltd., California, USA) for 2 hrs and then rinsed with 1× phosphate buffered saline three times for 5 mins each.

Techniques:

Journal: OncoTargets and therapy

Article Title: Roles Of EAAT1, DHFR, And Fetuin-A In The Pathogenesis, Progression And Prognosis Of Chondrosarcoma

doi: 10.2147/OTT.S222426

Figure Lengend Snippet: EAAT1, DHFR, and fetuin-A ROC curves for overall survival in CS patients (N = 80) from January 2011 to June 2015. AUCs for EAAT1 ( A ), DHFR ( B ), and fetuin-A ( C ) were 0.654, 0.638, and 0.670, respectively, indicating good predictive performance.

Article Snippet: Following a 20-min sitting in sodium citrate buffer (10 mM sodium citrate, 0.05% Tween 20, pH 6.0) at room temperature for 20 mins, the sections were incubated with rabbit anti-human EAAT1 [1:50], DHFR [1:50], or fetuin-A [1:50] antibodies (Boster Biological Technology Co., Ltd., California, USA) for 2 hrs and then rinsed with 1× phosphate buffered saline three times for 5 mins each.

Techniques: